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Introduction of Loeffler Serum Slope Loeffler Serum slope is used for the cultivation of Corynebacterium diphtheriae. It was originally devised by Loeffler and further modified by Perry and Petran and Buck. Loeffler serum slope enhances primary and secondary isolation and cultivation of fastidious pathogenic microorganisms especially from the nose and throat which also restores virulence and other identifying properties after they have been lost due to prolonged incubation or repeated subculturing. This Loeffler medium is also...

Introduction of Litmus Milk Test Litmus Milk Test needs a litmus milk medium and it is a liquid medium that helps to differentiate bacteria based on various metabolic reactions in litmus milk, including fermentation, reduction, clot formation, digestion, and the formation of gas. This medium is used primarily to differentiate members within the genus Clostridium and it also differentiates Enterobacteriaceae from other Gram-negative bacilli based on the ability of enteric bacilli to reduce litmus. It is...

Introduction of Eosin Methylene Blue (EMB) Agar EMB stands for Eosin Methylene Blue and EMB agar is a differential bacterial medium, which slightly inhibits the growth of Gram-positive bacteria and provides a color indicator distinguishing between organisms that ferment the lactose (e.g., E. coli, Klebsiella) and those that do not (e.g., Proteus, Salmonella, Shigella). This agar was originally devised by Holt Harris and Teague and further modified by Levine. The modified form is a combination of...