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Introduction of Ascospore Agar Ascospore Agar is recommended medium for the expression of ascospores and therefore it is useful for enrichment and detection of ascosporogenous yeasts. It is the modification of the McClary medium with the addition of potassium acetate in place of sodium acetate. Saccharomyces cerevisiae is the most common yeast used to show expression ascopores. Principle of Ascospore Agar Yeast extract and dextrose supply the nutrients required for the growth and also stimulate ascospore formation...

Uses of L-pyrrolidinyl-β-naphthylamide Test L-pyrrolidinyl-β-naphthylamide Test short form is PYR  test. This PYR test uses to identify the capability of organisms utilizing substrate, PYR by L-pyrroglutamyl amino-peptidase. Application of this test are as follows: Identification of Streptococcus pyogenes (PYR positive)from other beta-hemolytic Streptococci (Negative) Differentiation of Enterococcus species (PYR positive) from group D Streptococci (Streptococcus bovis, Streptococcus equinus) which are PYR negative. It is used in the identification of Escherichia coli (PYR Negative), separating it from other indoles positive, lactose positive,...

Introduction of 4-Methylumbelliferyl-β-D Glucuronide Test 4-Methylumbelliferyl-β-D Glucuronide Test short form is MUG test and it acts as a substrate for the organisms those having the enzyme β-glucuronidase. This enzyme is present in 97% of strains of E. coli. Organisms other than E. coli of the Enterobacteriaceae family  Salmonella, Shigella, and  Yersinia also possess the enzyme β-glucuronidase. This test uses for rapid identification of E. coli, the most common gram-negative rod seen in clinical specimens. Since verotoxin-producing E. coli strains...