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Introduction of Tryptone Water Tryptone water is used for the detection of indole production by coliforms ( E. coli, Klebsiella oxytoca). Principle of Tryptone Water Water is the source of hydrogen and oxygen. Sodium chloride is the source of electrolytes. Casein enzymic hydrolysate is a good substrate for indole production due to its high tryptophan content. Certain bacteria break down the amino acid tryptophan with the help of enzymes that mediate the production of indole by...

Introduction of SYBR Safe DNA Gel Stain SYBR Safe DNA Gel Stain name is a DNA stain but it also stains RNA. It is a highly sensitive stain for visualization of DNA in agarose or acrylamide gels. It is specifically formulated to be a less hazardous alternative to commonly using ethidium bromide that can utilize either blue light or UV excitation. It is beneficial for us for the following purposes- It reduces exposure to highly mutagenic...

Introduction of MR-VP Test MR-VP test stands for Methyl Red -Voges Proskauer Test. MR-VP test will be discussed in two tests separately. One for Methyl Red (MR) test while another for Voges Proskauer (VP)test. Introduction of MR Test MR test stands for Methyl Red test. It is used for enteric gram-negative rods, as part of identification to species level. MR test positive bacteria are Escherichia coli, Klebsiella ozanae, Klebsiella rhinoscleromatis while negative are  Klebsiella pneumoniae, Enterobacter species, etc. Principle of MR Test The methyl red (MR)...