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Trichomoniasis: Introduction, Symptoms, Risk factors, Lab diagnosis, Treatment and Prevention
Introduction of trichomoniasis Trichomoniasis is a sexually transmitted infection. A protozoan parasite, Trichomonas vaginalis is a causative agent of this infection that is very common. and easily treatable. T. vaginalis travels from person to person through genital contact during sex. In women, the organism causes an infection in the vagina, urethra, or both. In men, the infection only happens in the urethra. Once the infection begins, it can easily be spread through unprotected genital contact whereas it...
Acridine Orange Stain: Introduction, Principle, Procedure and Result Interpretation
Introduction of Acridine orange Acridine orange is a fluorochrome stain and uses the rapid identification of Trichomonas vaginalis, yeast cells, and clue cells in vaginal smears. It can also use to detect intracellular gonococci, meningococci, and other bacteria, particularly in blood cultures. [embed]https://www.youtube.com/watch?v=EczeAWwsoQw[/embed] Principle of Acridine orange Acridine orange is a fluorochrome that causes DNA to fluoresce green and RNA to fluoresce orange-red. Requirement for Acridine orange Acridine orange acid stain Alcohol saline solution physiological saline Test specimen Glass...
SARS-CoV-2 Genes detection using Real Time RT-PCR: Introduction, Principle, Procedure and Result Interpretation
Introduction of SARS-CoV-2 genes detection SARS-CoV-2 genes detection using real-time RT-PCR is both a screening and confirmatory test of COVID-19 disease and SARS-CoV-2 stands for Severe acute respiratory syndrome coronavirus. It is a causative agent of Coronavirus disease 2019 or COVID -19 is a viral disease. It is a positive-sense single-stranded RNA virus and strain was first discovered in Wuhan, China, o it is sometimes referred to as the “Wuhan virus” or “Wuhan coronavirus”. The virus is primarily spread between people through...