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RCM: Introduction, Principle, Composition, Preparation, Test Procedure, Result Interpretation, Uses and Keynotes
Introduction of Robertson’s Cooked Meat (RCM) Medium Robertson’s Cooked Meat (RCM) Medium is the recommended medium for the cultivation of anaerobic as well as aerobic bacteria. It is especially for pathogenic Clostridium species from clinical, food, and water samples. This can also be used as a maintenance medium for stock cultures of anaerobes as well as aerobes. RCM is also known as cooked meat broth (CMB) since it contains pieces of fat-free minced cooked meat of ox heart...
Thioglycollate Medium: Introduction, Composition, Preparation, Principle, Test Procedure, Result Interpretation, Uses and Keynotes
Introduction of Thioglycollate Medium Thioglycollate Medium is recommended for sterility testing of biologicals and for the cultivation of anaerobes, aerobes, and microaerophiles from pharmaceutical as well as clinical samples. Composition of Thioglycollate Medium Ingredients Gms / Litre Tryptone: 15.0 Yeast extract: 5.0 Dextrose (Glucose): 5.5 Sodium chloride(NaCl):2.5 L-Cystine: 0.5 Sodium thioglycollate: 0.5 Resazurin sodium: 0.001 Agar: 0.75 Final pH ( at 25°C): 7.1±0.2 Preparation of Thioglycollate Medium Suspend 2.975 grams in 100 ml distilled water or...
SDA: Introduction, Principle, Composition, Preparation, Colony Characteristics and Uses
Introduction of SDA SDA stands for Sabouraud Dextrose Agar and Sabouraud is the surname of creator Raymond Jacques Adrien Sabouraud a French physician born in Nantes. He formulated SDA in 1892 for culturing dermatophytes. SDA is the most common fungal medium to recover the growth of fungi in Mycology. It is different from bacterial medium due to having main two properties, comparatively low pH (5.6) and high concentration of sugar. It is useful for the isolation, cultivation, and...