Rapid Plasma Reagin (RPR) Test Card demonstrating RPR Test-It works on the principle of flocculation and flocculation is a special type of precipitation where precipitates remain suspended instead of sedimentation. This is the most common test in the clinical laboratory for screening of syphilis that is caused by the etiological agent Treponema pallidum. Principle of Rapid Plasma Reagin (RPR) Test In the Rapid Plasma Reagin test, the RPR antigen is mixed with unheated or heated serum...
Introduction of Real-Time RT-PCR in COVID-19 Detection SARS-CoV-2 genes detection using real-time RT-PCR is both a screening and confirmatory test of COVID-19 disease and SARS-CoV-2 stands for Severe acute respiratory syndrome coronavirus. It is a causative agent of Coronavirus disease 2019 or COVID -19 is a viral disease. It is a positive-sense single-stranded RNA virus and strain was first discovered in Wuhan, China, o it is sometimes referred to as the “Wuhan virus” or “Wuhan coronavirus”. The virus is primarily spread between...
History and Introduction of Mueller Hinton Agar (MHA) The name Mueller- Hinton agar(MHA) is from the surname of co-developers microbiologist John Howard Mueller and veterinary scientist Jane Hilton at Harvard University as a culture for gonococcus and meningococcus, who published the method in 1941. It is a non-selective and non-differential medium. In 1966, Bauer et al. adopted MHA for antimicrobial susceptibility testing. Later Muller- Hinton agar (MHA) was adopted as the common medium to use for routine antibiotics susceptibility testing...