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COVID19 PCR Test MCQs

COVID19 PCR Test MCQs are really useful since there are numerous laboratories busy in this COVID pandemic to diagnose SARS-CoV-2 ( Severe acute respiratory syndrome coronavirus) as a causative agent of  Coronavirus disease 2019 or COVID -19 is a viral disease whereas the provisional name 2019 novel coronavirus (2019-nCoV). It is a positive-sense single-stranded RNA virus and strain was first discovered in Wuhan, China, so it is sometimes referred to as the “Wuhan virus” or “Wuhan coronavirus”. Primary reservoir bat whereas an intermediate animal reservoir such as a pangolin is also thought to be involved in its introduction to humans. The virus is primarily spread between people through close contact and via respiratory droplets produced from coughs or sneezes. It mainly enters human cells by binding to the receptor angiotensin-converting enzyme 2 (ACE2). Thousands of laboratory personnel are involving in COVID PCR Laboratory. The set of COVID19 PCR Test MCQs is a simple form that creates enjoyable moments. 

Collection of COVID19 PCR Test MCQs

1. Prevention of contamination in PCR lab, the flow should be

• Unidirectional
• Bidirectional
• Non-directional
• Any of the above

2. As per current guidelines, positive samples of COVID-19 PCR should be stored at -80°C up to

• Fifteen days
• One month
• Two months
• Three months

3. How to optimize the use of PPE?

• Ensure rational use of PPE
• Minimize the use of PPE
• Co-ordinate PPE chain supply
• All of above

4. Which of the following is NOT a component of the Master mix?

• dNTPs
• Probes
• Template
• Taq-polymerase

5. If RNA extract cannot be immediately processed for PCR, it should be stored at,

• Room temperature
• 2-8° C
• -20° C
• -80° C

6. Extraction is a process to purify

• Proteins
• Nucleic acids
• A and B
• All of the above

7. Steps of extraction are :

• Binding/Lysis/Washing/Elution
• Lysis/Washing/Binding/Elution
• Lysis/Binding/Washing/Elution
• Washing/Lysis/Binding/Elution

8. Role of carrier RNA

• Enhanced binding of viral nucleic acid
• Reduced viral RNA recovery if not added
• A and B
• None of the above

9. In one-step PCR reaction…

• Reverse transcriptase is included in the same tube as PCR
• Reverse transcriptase is not included in the same tube as PCR
• DNA polymerase is included in the same tube as PCR
• DNA polymerase is not included in the same tube as PCR

10.  At what temperature should the samples (collected in VTM) be stored if there is a delay of more than 48 hours for processing or transportation

• 2-8° C
• -70 ° C
• In the freezer compartment
• At room temperature

11. The process of dividing the sample into various vials into small portions is known as:

• Extraction
• Archiving
• Aliquoting
• PCR

12. …is to maintain biological sample and their related information for the lab testing and research.

• Archiving
• Storage
• Collection
• Transportation

13. Workflow inside biosafety cabinet should be from

• Clean to dirty
• Dirty to clean
• Clean to clean
• Dirty to dirty

14. Full form of HEPA-

• High-efficiency performance air
• High extraction particulate air
• High-efficiency particulate air
• High extraction performance air

15. Which factor is/are important for obtaining the right results?

• Time and site of sample collection
• Method of collection
• Storage and transportation of samples
• All of the above
• None of the above

16. Oropharyngeal and nasopharyngeal swabs should be collected

• After antiviral therapy
• As soon as the symptoms appear
• Using cotton swabs
• All of the above

17. VTM stands for …

• Viral Team Master
• Viral Transport Master
• Viral Transport Media
• Virus Transport Media

18. Specimen data forms, letters and other types of information on the sample to be sent should be packed in…

• Primary container along with sample
• Secondary container
• Tertiary container
• Any of the above

Right Answers of COVID19 PCR Test MCQs

1. Unidirectional
2. Three months
3. All of above
4. Template
5. -20° C
6. Nucleic acids
7. Lysis/Binding/Washing/Elution
8. a and b
9. Reverse transcriptase is included in the same tube as PCR
10. -70 ° C
11. Aliquoting
12. Storage
13. Clean to dirty
14. High-efficiency particulate air
15. All of the above
16. Using cotton
17. Viral Transport Media,
18. Tertiary container