Tag: Preparation of PDA

PDA: Introduction, Principle, Composition, Test Procedure, Colony Characteristics and Uses

PDA: Introduction, Principle, Composition, Test Procedure, Colony Characteristics and Uses Introduction of PDA PDA stands for Potato Dextrose Agar and...

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Potato Dextrose Agar (PDA): Introduction, Principle, Composition, Test Procedure, Colony Characteristics and Uses

Potato Dextrose Agar contains dehydrated Potato Infusion and Dextrose. Potato infusion provides a nutrient base for luxuriant growth of most fungi where as Dextrose serves as a growth stimulant. Agar in the medium acts the solidifying agent. PDA has further modified incorporating agents like tartaric acid, Chloramphenicol  and Chlortetracycline. The incorporation of tartaric acid (TA) in the medium lowers the pH to 3.5 which inhibits bacterial growth. Chloramphenicol acts as a selective agent to inhibit bacterial overgrowth of competing microorganisms from mixed specimens, while permitting the selective isolation of fungi. Application of Chlortetracycline in PDA is  for the evaluation of yeast and mold from cosmetic products. Introduction of Potato Dextrose Agar (PDA) Potato Dextrose Agar ( PDA)...

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