Tag: Limitations of Deoxycholate Citrate Agar (DCA)

Deoxycholate Citrate Agar (DCA): Introduction, Principle, Composition, Test Procedure, Colony Characteristics and Uses

Deoxycholate Citrate Agar (DCA) is a  recommended medium for the isolation of enteric pathogens particularly Salmonella and Shigella species from clinical  as well food samples. DCA is a modification of Desoxycholate Agar formulated by Leifson. His original medium demonstrated improved recovery of intestinal pathogens from specimens containing normal intestinal flora by using citrates and sodium desoxycholate in specified amounts as inhibitors to gram-positive bacteria. Leifson modified his original medium by increasing the concentration of sodium citrate and sodium desoxycholate and found Desoxycholate Citrate Agar reliable for isolating many Salmonella and Shigella species. DCA  effectively isolates intestinal pathogens (Salmonella and Shigella species) by inhibiting coliforms and many Proteus species. Introduction of Deoxycholate Citrate Agar (DCA) Deoxycholate Citrate Agar (DCA)...

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