Endolimax nana in Saline Preparation : Introduction and Morphology
Endolimax nana in saline preparation
Endolimax nana in saline preparation showing plenty of binucleate and occasional mononucleate and quadrinucleate cysts as shown above picture.
Scientific classification of E. nana
- Domain: Eukaryota
- Phylum: Amoebozoa
- Subphylum: Conosa
- Class: Archamoebea
- Order: Pelobiontida
- Family: Mastigamoebidae
- Genus: Endolimax
- Species: E. nana
Introduction of Endolimax
Endolimax is a protozoan parasite that is found in the intestines. E. nana found in us are originally thought to be non-pathogenic, studies suggest it can cause intermittent or chronic diarrhea. It forms cysts with four nuclei that exocyst in the body and become trophozoites. E. nana nuclei have a large endosome somewhat off-center and small amounts of visible chromatin or none at all.
Morphology of Endolimax
It consists of cyst and trophozoite.
Cysts: They are small, with a spherical to ellipsoidal shape. Mature cysts contain four nuclei; immature cysts have one and two nuclei inside them. These cysts measure 5–10 um, with a usual range of 6–8 um.
Trophozoites: This stage is small, measuring 6–12 µm, with an average range of 8–10 um. Living trophozoites are sluggish and generally non-progressive. The single nucleus sometimes is visible in unstained preparations as shown in the video.
Further Readings
- Molecular Medical Parasitology. Editors: J. Joseph Marr, Timothy W. Nilsen, and Richard W. Komuniecki, Publisher Academic Press, an imprint of Elsevier Science.
- https://wikimili.com/en/Endolimax
- Medical Parasitology by Abhay R. Satoskar, Gary L. Simon, Peter J. Hotez and Moriya Tsuji
- Atlas of Medical Helminthology and protozoology -4th edn -P.L. Chiodini, A.H. Moody, D.W. Manser
- Merkell and Voge’s medical parasitology
9th edition. - Parasitology: 12th edition
By K. D. Chatterjee - District laboratory practice in Tropical countries –Part-I.
By Monica Chesbrough. - Isenberg clinical microbiology procedures Handbook
2nd edition. Vol. 2 - Atlas of Human Parasitology, Lawrence R Ash, Thomas C. Orihel, 3 rd ed, Publisher ASCP Press, Chicago.
- https://pubmed.ncbi.nlm.nih.gov/10605115/