Staphylococcus epidermidis- Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes

Staphylococcus epidermidis- Introduction, Morphology, Pathogenicity, Lab Diagnosis, Treatment, Prevention, and Keynotes


Staphylococcus epidermidis is a Gram-positive, coagulase-negative bacterium that belongs to the Staphylococcus genus. It is one of the most common members of the human skin microbiota and is typically found on the skin and mucous membranes. Staphylococcus epidermidis is considered a commensal bacterium, which means that it usually coexists harmlessly with its human host. However, under certain conditions, it can become an opportunistic pathogen and cause infections, particularly in individuals with compromised immune systems or those with implanted medical devices.

Here are some key characteristics and features of Staphylococcus epidermidis:

  1. Microbial Habitat: Staphylococcus epidermidis is primarily found on the skin, but it can also colonize other areas of the body, such as the mucous membranes and the gastrointestinal tract. It is a normal component of the human microbiome.
  2. Gram-Positive: It is a Gram-positive bacterium, which means that it has a thick peptidoglycan cell wall that retains a purple stain in the Gram staining process.
  3. Coagulase-Negative: Staphylococcus epidermidis is coagulase-negative, which distinguishes it from more pathogenic staphylococcal species like Staphylococcus aureus, which is coagulase-positive.
  4. Biofilm Formation: One of the notable characteristics of Staphylococcus epidermidis is its ability to form biofilms on surfaces, including medical devices such as catheters and prosthetic joints. Biofilms provide protection and can make infections difficult to treat.
  5. Opportunistic Infections: While Staphylococcus epidermidis is typically harmless, it can cause infections when it gains access to the bloodstream or deeper tissues, often in healthcare settings. These infections can include bloodstream infections (bacteremia), endocarditis (inflammation of the heart’s inner lining), urinary tract infections, and infections associated with indwelling medical devices.
  6. Antibiotic Resistance: Many strains of Staphylococcus epidermidis have developed resistance to antibiotics, making treatment challenging. Methicillin-resistant Staphylococcus epidermidis (MRSE) is an example of a resistant strain.
  7. Diagnostics: Identification of Staphylococcus epidermidis typically involves laboratory culture and biochemical tests. Molecular techniques like polymerase chain reaction (PCR) and DNA sequencing can also be used for precise identification.
  8. Prevention and Control: Preventing Staphylococcus epidermidis infections often involves proper hygiene, especially in healthcare settings. Strict adherence to infection control measures and the judicious use of antibiotics are important in managing these infections.


The morphology of Staphylococcus epidermidis refers to its physical characteristics and structure. Here is an overview of the morphology of Staphylococcus epidermidis:

  1. Cell Shape: Staphylococcus epidermidis cells are typically spherical or round in shape. They are classified as cocci, which are a type of bacterial shape characterized by a spherical or ovoid structure.
  2. Cell Arrangement: Staphylococcus species, including Staphylococcus epidermidis, are known for their characteristic arrangement in grape-like clusters. These clusters are a result of cell division in multiple planes, which gives the appearance of clusters under the microscope.
  3. Size: The size of individual Staphylococcus epidermidis cells can vary slightly, but they are generally small, with a diameter of approximately 0.5 to 1.0 micrometers (µm). These cells are relatively small compared to some other bacterial species.
  4. Gram Staining: Staphylococcus epidermidis is Gram-positive, which means that it retains the purple stain in the Gram staining process. This indicates that it has a thick layer of peptidoglycan in its cell wall, which contributes to its ability to retain the stain.
  5. Cell Wall: Like other Gram-positive bacteria, S. epidermidis has a thick peptidoglycan cell wall, which provides structural support and rigidity to the cell. The cell wall also contains teichoic acids.
  6. Capsule: Some strains of S. epidermidis may produce a slimy capsule composed of polysaccharides. This capsule can enhance the bacterium’s ability to adhere to surfaces and may contribute to its pathogenicity.
  7. Flagella and Motility: Staphylococcus epidermidis is generally non-motile, meaning it lacks flagella and does not have the capability to move actively in liquid environments. This non-motility is in contrast to some other bacterial species that possess flagella for motility.
  8. Biofilm Formation: S. epidermidis is known for its ability to form biofilms, which are communities of bacterial cells embedded in a matrix of extracellular substances. Biofilms provide protection and help the bacteria adhere to surfaces, which is a key factor in the bacterium’s pathogenicity.


S. epidermidis is typically considered a commensal bacterium, meaning it coexists harmlessly with its human host as part of the normal skin and mucous membrane microbiota. However, under certain conditions, it can become an opportunistic pathogen and cause various infections, particularly in individuals with compromised immune systems or those with implanted medical devices. Here’s an overview of the pathogenicity of Staphylococcus epidermidis:

  1. Medical Device-Related Infections: S. epidermidis is a leading cause of infections associated with indwelling medical devices such as catheters, prosthetic joints (e.g., hip and knee replacements), pacemakers, and artificial heart valves. It has a particular affinity for adhering to the surfaces of these devices and forming biofilms. Once a biofilm is established, it becomes difficult for the immune system and antibiotics to eradicate the infection.
  2. Biofilm Formation: The ability of S. epidermidis to form robust biofilms is a key factor in its pathogenicity. Biofilms protect the bacteria from the host’s immune response and hinder the penetration of antibiotics. As a result, infections involving these biofilms can be persistent and challenging to treat.
  3. Infective Endocarditis: Staphylococcus epidermidis can cause infective endocarditis, which is an infection of the inner lining of the heart (endocardium) or the heart valves. This condition can be life-threatening, particularly in individuals with pre-existing heart conditions.
  4. Bacteremia: S. epidermidis can enter the bloodstream, leading to bacteremia (the presence of bacteria in the blood). While bacteremia itself is a serious condition, it can also result in secondary infections in various organs, such as the bones (osteomyelitis), joints (septic arthritis), or other tissues.
  5. Urinary Tract Infections: In rare cases, S. epidermidis can cause urinary tract infections, especially in individuals with urinary catheters.
  6. Intraocular Infections: It has been associated with post-surgical endophthalmitis, which is an infection within the eye following eye surgery, such as cataract surgery. This can lead to vision loss if not promptly treated.
  7. Antibiotic Resistance: Many strains of S. epidermidis have developed antibiotic resistance, including resistance to methicillin (methicillin-resistant Staphylococcus epidermidis or MRSE). This resistance can complicate treatment and limit the available therapeutic options.

It’s important to note that not all strains of Staphylococcus epidermidis are equally virulent, and the likelihood of infection depends on various factors, including the patient’s health, the presence of medical devices, and the specific strain of the bacterium. In healthcare settings, strict adherence to infection control measures and the judicious use of antibiotics are crucial for preventing and managing S. epidermidis infections, particularly those associated with medical devices.

Lab Diagnosis

The laboratory diagnosis of Staphylococcus epidermidis involves several steps to identify and confirm the presence of this bacterium in a clinical sample. This is important for diagnosing infections and guiding appropriate treatment. Here are the key laboratory techniques and steps involved in diagnosing Staphylococcus epidermidis:

  1. Sample Collection: The first step in diagnosis is collecting a clinical specimen from the patient. Depending on the suspected infection site, samples can include blood, urine, wound swabs, or samples from infected medical devices (e.g., catheters).
  2. Microscopy: The clinical specimen is typically examined under a microscope to identify the presence of bacteria. Gram staining is commonly used to determine if the bacteria are Gram-positive cocci, which is characteristic of Staphylococcus species.
  3. Culture: The next step is to culture the specimen on appropriate agar plates. S. epidermidis grows well on general-purpose agar plates like blood agar or Mannitol Salt Agar (MSA). On MSA, Staphylococcus epidermidis typically appears as small, whitish colonies. It is coagulase-negative, which can help differentiate it from coagulase-positive Staphylococcus aureus.
  4. Biochemical Tests: Biochemical tests are used to confirm the identification of Staphylococcus epidermidis. Common tests include catalase and coagulase tests. S. epidermidis is catalase-positive (it produces bubbles when hydrogen peroxide is added) and coagulase-negative (it does not produce coagulase).
  5. Molecular Identification: In some cases, molecular techniques like polymerase chain reaction (PCR) and DNA sequencing may be used for precise identification of S. epidermidis. These methods can detect specific genetic markers unique to this bacterium.
  6. Antimicrobial Susceptibility Testing: To guide treatment decisions, antimicrobial susceptibility testing is performed to determine which antibiotics are effective against the isolated strain. This helps identify any antibiotic resistance patterns.
  7. Biofilm Detection: If the infection is associated with a medical device, such as a catheter, additional tests may be conducted to detect biofilm formation by Staphylococcus epidermidis. Techniques like the Congo red agar method or crystal violet staining can assess the presence of biofilms.
  8. Serotyping: In research or epidemiological studies, serotyping of S. epidermidis strains may be performed to further characterize isolates based on specific surface antigens.
  9. Genomic Typing: Whole-genome sequencing (WGS) and other genomic typing methods can provide detailed information about the genetic makeup of S. epidermidis isolates, helping to track outbreaks and study the bacterium’s genetic diversity.

It’s important to note that the choice of diagnostic tests and techniques may vary depending on the clinical presentation and the resources available in the laboratory. Rapid and accurate diagnosis of Staphylococcus epidermidis infections is crucial for appropriate patient management and the prevention of complications, especially in cases involving medical device-related infections or systemic infections like bacteremia.


The treatment of S. epidermidis infections typically involves the use of antibiotics, but it can be challenging due to the bacterium’s ability to form biofilms and develop antibiotic resistance. The choice of antibiotics and the duration of treatment depend on the specific clinical presentation, the site of infection, and the results of antibiotic susceptibility testing. Here are some general considerations for the treatment of S. epidermidis infections:

  1. Antibiotic Selection: The choice of antibiotics should be guided by the results of antimicrobial susceptibility testing, which identifies which antibiotics are effective against the particular strain of S. epidermidis. Commonly used antibiotics for treating Staphylococcus epidermidis infections include:
    • Vancomycin
    • Teicoplanin
    • Linezolid
    • Daptomycin
    • Rifampin (often used in combination with other antibiotics)
    • Trimethoprim-sulfamethoxazole (TMP-SMX)
    • Ciprofloxacin
  2. Biofilm-Associated Infections: S. epidermidis is known for its ability to form biofilms, especially on medical devices. Biofilms can be highly resistant to antibiotics. In cases of device-related infections, it is often necessary to remove or replace the infected device (e.g., catheter, prosthetic joint) in addition to antibiotic therapy.
  3. Duration of Treatment: The duration of antibiotic treatment varies depending on the type and severity of the infection. Short courses of antibiotics may be sufficient for minor skin or soft tissue infections, while more complex infections, such as endocarditis or osteomyelitis, may require prolonged treatment courses, often lasting weeks to months.
  4. Monitoring: Patients with S. epidermidis infections should be closely monitored for clinical improvement. Blood tests, imaging, and other diagnostic tools may be used to assess the progress of treatment and the resolution of the infection.
  5. Infection Control Measures: In healthcare settings, strict infection control measures are crucial to prevent the spread of Staphylococcus epidermidis infections, especially in cases of outbreaks or clusters. This includes proper hand hygiene, use of barrier precautions, and adherence to infection prevention protocols.
  6. Prevention: Preventing S. epidermidis infections is essential, particularly in healthcare settings. Strategies include using sterile techniques during medical procedures, minimizing the use of unnecessary medical devices, and following best practices for catheter care and surgical site preparation.
  7. Antibiotic Resistance: Be aware of antibiotic resistance patterns in S. epidermidis. Infections caused by multidrug-resistant or methicillin-resistant strains may require specialized treatment regimens.

It’s important to note that Staphylococcus epidermidis is often part of the normal human skin and mucous membrane microbiota, and not all strains are pathogenic. In some cases, especially with minor skin colonization, treatment may not be necessary. The decision to treat should be based on clinical judgment and the presence of signs and symptoms of infection.

Treatment should always be overseen by a healthcare provider, and patients should complete the full course of antibiotics as prescribed to ensure the infection is fully eradicated and to minimize the risk of antibiotic resistance.


Preventing S. epidermidis infections, especially those associated with healthcare settings and medical devices, is crucial to reduce the risk of complications and the spread of antibiotic-resistant strains. Here are some key prevention strategies:

  1. Hand Hygiene: Proper hand hygiene is one of the most effective ways to prevent the spread of Staphylococcus epidermidis and other pathogens. Healthcare workers should practice regular handwashing with soap and water or use hand sanitizers containing at least 60% alcohol.
  2. Infection Control Measures: Implement and adhere to strict infection control measures in healthcare facilities. This includes wearing appropriate personal protective equipment (e.g., gloves, gowns, masks) when handling patients and following established protocols for preventing healthcare-associated infections.
  3. Aseptic Techniques: Use aseptic techniques during medical procedures, including the insertion and care of indwelling medical devices like catheters and intravenous lines. Ensure that all equipment and supplies used are sterile.
  4. Catheter Care: Follow best practices for catheter care to prevent catheter-associated infections. This includes proper insertion techniques, regular assessment for signs of infection, and timely removal of catheters when they are no longer needed.
  5. Surgical Site Preparation: Ensure that surgical sites are properly prepared before procedures to reduce the risk of surgical site infections. This may include preoperative antimicrobial prophylaxis, maintaining a sterile environment, and adhering to surgical hand hygiene practices.
  6. Antibiotic Stewardship: Promote the responsible use of antibiotics to prevent the development and spread of antibiotic-resistant strains of Staphylococcus epidermidis. Antibiotics should only be prescribed when medically necessary and according to established guidelines.
  7. Device-Associated Infection Prevention: For patients with implanted medical devices (e.g., prosthetic joints, pacemakers), follow strict infection prevention protocols, including proper surgical techniques and postoperative care to reduce the risk of device-related infections.
  8. Environmental Cleaning: Maintain a clean healthcare environment by implementing regular and thorough cleaning and disinfection protocols. Focus on high-touch surfaces and equipment.
  9. Surveillance and Outbreak Investigations: Implement surveillance systems to monitor and detect clusters or outbreaks of Staphylococcus epidermidis infections in healthcare settings. Promptly investigate and control outbreaks to prevent further spread.
  10. Patient Education: Educate patients and their families about the importance of hand hygiene and infection prevention measures. Encourage patients to be actively involved in their care and to speak up if they observe lapses in infection control practices.
  11. Proper Dressing and Wound Care: For patients with wounds or surgical incisions, ensure that dressings are applied and changed according to established protocols. Proper wound care is essential to prevent infection.
  12. Isolation Precautions: Implement isolation precautions, such as contact precautions, for patients known or suspected to be colonized or infected with multidrug-resistant strains of S. epidermidis.
  13. Healthcare Worker Vaccination: Ensure that healthcare workers are up-to-date with vaccinations, including those for preventable infections such as influenza and hepatitis B, to reduce the risk of transmission.


Here are some key points to remember about Staphylococcus epidermidis:

  1. Normal Skin Flora: S. epidermidis is a commensal bacterium commonly found on the skin and mucous membranes of humans. It is part of the normal skin microbiota.
  2. Gram-Positive Cocci: S. epidermidis is a Gram-positive bacterium, appearing as spherical cells that typically arrange in grape-like clusters.
  3. Coagulase-Negative: It is coagulase-negative, which distinguishes it from the more pathogenic Staphylococcus aureus, which is coagulase-positive.
  4. Biofilm Formation: It is known for its ability to form biofilms, particularly on medical devices. Biofilms protect the bacteria and make infections difficult to treat.
  5. Opportunistic Pathogen: While usually harmless, it can cause infections in certain conditions, especially in individuals with weakened immune systems or those with implanted medical devices.
  6. Infections: Common infections associated with S. epidermidis include catheter-related bloodstream infections, endocarditis, urinary tract infections, and infections of surgical sites and prosthetic devices.
  7. Antibiotic Resistance: Many strains of S. epidermidis have developed resistance to antibiotics, making treatment challenging.
  8. Diagnosis: Laboratory diagnosis involves microscopy, culture, biochemical tests (e.g., catalase and coagulase tests), and antimicrobial susceptibility testing.
  9. Treatment: Treatment involves antibiotics, with the choice based on susceptibility testing. Removal of infected medical devices may be necessary. Prolonged treatment may be required for severe infections.
  10. Prevention: Preventing Staphylococcus epidermidis infections involves proper hand hygiene, infection control measures, aseptic techniques, and responsible antibiotic use, especially in healthcare settings.
  11. Patient Education: Patients should be educated about infection prevention measures, and healthcare workers should follow strict infection control protocols.
  12. Vaccination: Healthcare workers should be up-to-date on vaccinations to reduce the risk of transmitting infections to vulnerable patients.
  13. Surveillance: Healthcare facilities should implement surveillance systems to monitor and detect outbreaks of S. epidermidis infections.
  14. Environmental Cleaning: Regular and thorough cleaning and disinfection of healthcare environments are essential.
  15. Multidisciplinary Approach: Preventing S. epidermidis infections requires a coordinated effort involving healthcare providers, infection control teams, patients, and their families.

Further Readings

  1. Scientific Journals:
    • Explore scientific journals like “Journal of Clinical Microbiology,” “Journal of Medical Microbiology,” “Clinical Infectious Diseases,” and “Antimicrobial Agents and Chemotherapy” for research articles, reviews, and case studies related to Staphylococcus epidermidis.
  2. Books:
    • “Staphylococcus: Genetics and Physiology” by G. O. Oscherwitz and A. L. Demain provides an in-depth exploration of various Staphylococcus species, including Staphylococcus epidermidis.
  3. Clinical Microbiology and Infectious Disease Textbooks:
    • Textbooks like “Clinical Microbiology Made Ridiculously Simple” by Mark Gladwin and Bill Trattler or “Mandell, Douglas, and Bennett’s Principles and Practice of Infectious Diseases” by John E. Bennett and Raphael Dolin contain valuable information on microbiology and infectious diseases, including Staphylococcus species.
  4. Online Resources:
    • Websites and online resources from reputable health organizations and academic institutions, such as the Centers for Disease Control and Prevention (CDC), the World Health Organization (WHO), and university microbiology departments, often have information on S. epidermidis and related topics.
  5. Review Articles:
    • Review articles in scientific journals can provide comprehensive overviews of current research and knowledge on Staphylococcus epidermidis. Look for reviews in journals like “FEMS Microbiology Reviews” or “Microbiology and Molecular Biology Reviews.”
  6. Research Databases:
    • Explore research databases like PubMed, Google Scholar, and Web of Science to find recent research papers, reviews, and articles related to S. epidermidis.
  7. Medical and Microbiology Conferences:
    • Proceedings and presentations from medical and microbiology conferences often contain valuable insights and research findings related to Staphylococcus epidermidis and healthcare-associated infections.
  8. Online Courses and Educational Materials:
    • Universities and educational institutions may offer online courses and educational materials related to microbiology, infectious diseases, and infection control that cover Staphylococcus epidermidis.
  9. Microbiology Textbooks:
    • Microbiology textbooks, such as “Microbiology: An Introduction” by Gerard J. Tortora and Berdell R. Funke, can provide fundamental information on microbiology, including Staphylococcus species.
  10. Publications by Microbiology Research Institutions:
    • Research institutions specializing in microbiology, such as the American Society for Microbiology (ASM), often publish journals, newsletters, and educational materials that cover Staphylococcus epidermidis and related topics.
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