Klebsiella is from the surname of German-Swiss microbiologist Edwin Klebs (1834–1913). Klebsiella is a genus of Gram-negative, oxidase-negative, fimbriated, non -motile, non-sporing rod-shaped bacteria with a prominent polysaccharide-based capsule and having size o,f 0.5 -0.8 µm wide to 1 -2 µm. They are found everywhere in nature and also in bacterial flora in our intestines. Klebsiella pneumoniae scientific classification is as follows
It may cause severe bronchopneumonia, urinary tract infections, nosocomial infections, wound infections, septicemia, meningitis, and rarely diarrhea too.
Klebsiella pneumoniae isolated from septicemic patient blood culture; on the basis of the following tests:-
Blood culture bottle incubated into Bactec.
After 23 hours of incubation, Bactec indicated positive.
The positive bottle was then sub-cultured into solid media Chocolate agar, blood agar, and MacConkey agar.
After overnight incubation, growth was seen in all three plates.
On MacConkey agar, the colonies appeared large, mucoid, and pink in color.
The mucoid nature of colonies is due to capsular material produced by the organisms.
Short thick Gram-negative rods
Catalase test -Positive
Nitrate reduction test- Reduced to nitrite
No hydrogen sulfide (H2S) production
Production of gas
Motility Indole Urease (MIU) test
Non-motile because of only strict growth around the stab line
Urea hydrolyzation test– positive
Note: Klebsiella oxytoca is indole positive.
Fermentation of sugars (glucose, lactose, and mannitol )- acid and gas
Methyl red test-Negative
Lysine decarboxylase test-Positive
The antibiotic sensitivity test was also suggestive for Klebsiella pneumoniae because of ampicillin resistance which is intrinsically resistant.
Clinical isolates of Klebsiella pneumoniae are resistant to a wide range of antimicrobial agents like ampicillin, Amoxycillin, and carbenicillin. This resistance is due to the R plasmid present in these organisms. Organisms are usually sensitive to cephalosporins, trimethoprim, nitrofurantoin, amoxicillin-clavulanic acid, and aminoglycosides. However, plasmid-mediated resistance to gentamycin and various cephalosporins has been reported in hospital strains.
Klebsiella pneumoniae -introduction, pathogenesis, lab diagnosis and treatment
#K. pneumoniae growth on NA, BAP & MAC and its biochemical test-
#K. pneumoniae glucose utilization test using Andrade’s indicator-
# K. pneumoniae under Microscope ||K. pneumoniae Gram Stain
#K. pneumoniae growth on MacConkey agar, blood agar, and chocolate agar-
#Staphylococcus aureus, E. coli, and K. pneumoniae on growth on various media