Spot test in Parasitology: Common Parasites and Their Images with Salient Features

Spot test in Parasitology

Introduction of Spot test in Parasitology

‘Spot test in Parasitology’ is a common spot test in both bachelors as well as master degree programs i.e. B.Sc. MLT/ BMLT or MBBS or M.Sc. MLT/ M.Sc. Clinical/ Medical  Microbiology, etc.  This collection of spots makes your examination easy.

Egg of roundworm
Egg of roundworm

Main features

  • Bile stained
  • Scientific name for roundworm is Ascaris lumbricoides.
  • Route of  infection by ingestion
  • Infective form: embryonated egg
Egg of hookworm
Egg of hookworm

Salient Features

  1. The egg is not bile-stained.
  2. Scientific names for hookworm are Ancylostoma duodenale and Necator americanus
  3. Infective agent: Filariform larva
  4. Portal of entry: Through the skin.
Egg of Enterobious vermicula
Egg of Enterobious vermicularis

Salient features

  1. The egg is not bile stained
  2. Planoconvex in shape.
  3. Common names: Threadworm, pinworm, seat worm
  4. Clinical features: Pruritus ani (anal itch)
Hymenolypsis (now called Vampirolepsis) nana (H nana)- egg showing hooklets
Hymenolypsis (now called Vampirolepsis) nana (H nana)- egg showing hooklets

Main features

  1. The egg is not bile stained.
  2. It contains an oncosphere with three pairs of hooklets.
  3. Route of infection: By ingestion of food contaminated with eggs.
Trichuris trichura or whip worm egg
Trichuris trichura or whipworm egg

Principal  features

  1. The egg is bile stained
  2. Barrel-shaped with a mucous plug at each pole.
  3. Route of infection: By ingestion of embryonated eggs.
Cyst and trophozoite of Entamoeba histolytica or Amoeba
Cyst and trophozoite of Entamoeba histolytica or Amoeba

Salient features

  1. The cyst is quadrinucleate i.e contains four nuclei.
  2. It has a central karyosome present in the nucleus.
  3. Route of infection: By ingestion of water and food containing cysts.
  4. Diseases produced: Amoebic dysentery, Extraintestinal amoebiasis.
  5. The liver is the most commonly involved organ in extraintestinal amoebiasis.
Giardia lamblia Cysts in iodine wet mount
Giardia lamblia Cysts in iodine wet mount

Salient features

  1. The cyst is oval in shape.
  2. It contains four nuclei.
  3. The axostyles lie diagonally, forming a dividing line within the cyst wall.
  4. Route of infection: By ingestion of cysts.
  5. G. lamblia infection causes malabsorption.

Contd…

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