Egg of Taenia in Fecal Specimen: Identification Features and Wet Mount Preparation
Egg of Taenia species in fecal specimen
The above picture is showing an egg of Taenia species or tapeworm having embryophore, hooklets, oncosphere in stool saline wet mount.
Spherical in shape
Size- diameter 31-43µm
Brown color due to bile stained
It does not float in a saturated common salt solution.
Oncosphere: 14-20 µm in diameter, shows 3 pairs of hooklets.
There is little difference between normal and physiological saline. Physiological saline is 0.85% NaCl whereas normal saline is 0.9% NaCl.
Gram’s iodine is not applicable for staining parasitic organisms and for this D’Antoni’s iodine uses.
Oil immersion examination is also preferred in parasitology for the permanent stained smear of parasites.
Intestinal protozoa can not conform on the basis of a wet mount alone and thus permanent stained smears require to confirm the specific identification of suspected organisms.
Principle of the Saline Wet Mount of Stool
Saline wet mount preparation for stool is the simplest and basic method of analyzing a stool specimen in coprology (study of feces). It utilizes a physiological saline solution (0.85% NaCl ) as an isotonic media to maintain the cellular structure of the various organisms that are found in stool and that we like to examine.
Requirements for Saline Wet Mount
Physiological saline ( 0.85% NaCl)
Sterile bamboo sticks or low cone on the end of a wooden applicator stick