Introduction of Cellotape Method for Fungus Odervation
Cellotape Method for Fungus Observations is an alternative method for skin scraping. It is also an older technique of fungus observation directly from the skin infection. This method is especially suitable for Pityriasis Versicolor (fungal infection).
Test Requirements for Cellotape Method for Fungus Observation
Follow test requirements are needed to procced cellotape method for fungus observation-
- Suspected patient
- Cellotape
- Normal saline
- Sterile Cotton swab
- Eosin stain
- Clean and grease-free Glass slides
- Forceps
- Timer
- Microscope
Test Procedure of Cellotape Method for Fungus Observation
- Choose the patch of the infected skin area. Clean with saline.
- Apply a cotton swab soaked in eosin solution to moisten and stain the area.
- Cut a piece of cellotape and apply it firmly over the affected surface of the skin and leave for 5 minutes.
- Stick the tape on the skin and press firmly using the finger or a glass rod.
- Pull the adhesive tape away with forceps. Press it at once on the slide, sticky side down.
- Examine with 10X objective until the cluster of large granules (the spores) are seen and then move towards the 40X objective. You may also shift to 100X objective using cedarwood oil for detailed morphology.
Result Interpretation of Cellotape Method for Fungus Observation.
- The background is pink and the spores are white.
- The spores appear as a bunch or cluster, round or slightly rectangular 3- 8μm ×5μm in diameter, its cell wall is thick, and sometimes budding cells can be seen.
- Mycelium filaments appear long, bent, and twisted. 20- 40μm ×5μm. It resembles like finger and has branches.
- Look for the typical morphology of the dermatophytes and yeast etc.
- The typical yeasts are and short hyphae are seen easily.
Keynotes on Cellotape Method for Fungus Observation
- It is easy to transport the specimens.
- This technique is especially suitable for Pityriasis Versicolor. It is, sometimes called tinea versicolor and it is a common fungal infection that causes small patches of skin to become scaly and discolored.
- Malassezia is the etiological agent of Pityriasis versicolor..