Microbial Art: Introduction, Requirements, Making Procedure, Uses

Microbial Art: Introduction, Requirements, Making Procedure, Uses and Keynotes

Introduction of Microbial Art

Agar art or microbial art or germ art is the same thing in which artwork is created by culturing microorganisms in certain patterns. The microbes used can be bacteria, yeast fungi, or protists (less commonly). The microbes can be chosen for their natural colors. Organisms most commonly used and available are Staphylococcus aureus, Pseudomonas aeruginosa , Serratia marcescens as shown above picture. Painting with bacteria isn’t entirely new because Sir Alexander Fleming, the scientist responsible for discovering penicillin in the 1920s, began making “germ paintings” of bacteria during his career as a microbiologist. Similarly, Roger Tsien (biochemist) won the 2008 Nobel prize in chemistry for his contributions to the knowledge of green fluorescent protein (GFP), and using this GFP, Nathan Shaner (researcher) made a 2006 microbial artwork of a San Diego beach scene.

Requirements for Agar Art with Living Microbes

Agar plate
Microorganisms ( microbes):

Bacillus subtilis (cream to brown),
Chromobacterium violaceum (violet)
Escherichia coli (colorless)
Micrococcus luteus (yellow)
Micrococcus roseus (pink)
Proteus mirabilis
Pseudomonas aeruginosa strains having variuous pigments i.e. pyocyanin, pyoverdin, prorubrin and pyomelanin
Serratia marcescens (pink or orange)
Staphylococcus aureus (yellow)
Vibrio fischeri (bioluminescent)
Yeast species – which are fungi – used include Saccharomyces cerevisiae (yellow-white)
Aspergillus flavus (yellow-green spores)
Aspergillus ochraceus (yellow)
Aureobasidium pullulans (black)
Candida albicans (whitish buff)
Candida species (whitish)
Cladosporium herbarum (brown to black)
Cladosporium resinae
Epicoccum nigrum (yellow, orange, red, brown, and black),
Fusarium species
Rhodotorula species and Scopulariopsis brevicaulis
Protist species used include Euglena gracilis (photosynthetic, green) and
Physarum polycephalum (yellow-green)

Inoculating loop
Bunsen burner
Incubator

Procedure of Microbial Art

Before starting agar art with living microbes, keep all the following points in your mind-

Start planning early
Keep an eye out for colorful bacteria
Paint with a steady hand
Be ready to experiment
keep experimenting
Don’t give up

Allow the plates to warm at room temperature, and the agar surface to dry before inoculating.
Fill the portrait with colorful organisms.
Incubate the agar plate.
Observe the agar plate.

Uses of Microbial Art

You can use microbial art in the ‘ASM Agar Art Contest’.
You can also put agar art in your gallery or your laboratory to build your self-confidence as a creator.

Keynotes on Microbial Art

You can uses microbes as your interest.
Try to use normally non-pathogenic organisms.
Follow usual guidelines for all contaminated waste.
Take safety precautions during experiments.
If you are wording in the microbiology laboratory and thinking about agar art, store the colorful organisms.
For more details, watch the videos as shown below-

#Agar art with living microbes preparation tips and its importance-

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