Fungus Preservation Techniques: Introduction, Types and Detals About Sterile Water Preservation Technique

Fungus Preservation Techniques: Introduction, Types and Detals About Sterile Water Preservation Technique

Fungus Preservation Techniques

The main objectives of fungal culture are to grow the organisms, isolate the organisms, and if necessary preserve the organisms. A fungal culture is a genetic resource and thus fungus preservation techniques are applicable for the following purposes;

  • Germplasm conservation
  • Developmental studies
  • Industrial Application
  • Research programs

Types of Fungus Preservation Techniques

Fungal culture preservation techniques are as follows-

  1. Periodic serial subcultures
  2. Sterile water
  3. Sterile mineral oil overlay
  4.  Sterile soil or sand
  5. Silica gel
  6. Drying on organic substrates
  7. L-drying
  8. Mechanical freezers
  9. Lyophilization

Sterile Water Preservation Technique

Sterile Water Preservation Technique is recommended as easy, cheap, and satisfactory for the preservation of most species of fungi.

Requirements for Sterile Water Preservation Technique

  • Screwcap tubes
  • Distilled water
  • Fungal isolates
  • Inoculating loop/wire
  • Bunsen burner
  • Biosafety cabinet (BSC)
  • Tube rack

Procedure of Sterile Water Preservation Technique

  1. Take sterile screw cap tube with sterile distilled water.
  2. Submerge the fungal isolate into a  screw cap tube.
  3. Store it at room temperature (25-28 ° C).

Subculturing from the water

  1. Clean the screw cap with 70% ethanol before unscrewing them.
  2. Transfer a fragment from the tube to culture media.
  3. Incubate the culture media at room temperature (24-28° C) between 5-30 days depending on the nature of organisms.
  4. Perform LPCB preparation from culture and you may also use slide cultures for better expression of morphological features of fungi.

Note-Only performs these types of activities in the biosafety cabinet.

Keynotes on Fungus Preservation Techniques

  1. Preservation of fungal cultures in sterile distilled water is also called Castellani’s method that has been endorsed as a method ensuring high rates of fungal viability, purity and stability, low costs, and great simplicity where facing economic difficulties.
  2. Numerous fungi will survive for a decade or more in sterile water.
  3. There is no ideal technique of preservation appropriate for all laboratories and applicable to all fungi.
  4. It is noteworthy that all of these approaches are more expensive as compare to sterile water which can significantly reduce culture storage expenses.
  5. Liquid nitrogen (LN2) freezers- It is beneficial for the following purposes-√Unexcelled for the duration of storage

√Long-term viability is maximized

√All types of culturable fungi can be stored.

Further Reading on Fungus Preservation Techniques

  • https://hardydiagnostics.com/wpcontent/uploads/2016/05/Saving-fungal-Cultures-Sterile-Water-By-Harris.pdf
  • https://www.researchgate.net/publication/20359358_Preservation_of_fungi_in_water_Castellani_20_years
  • https://www.medigraphic.com/cgi-bin/new/resumenI.cgi?IDARTICULO=47061
  • https://www.biomed.cas.cz/ccbas/Methods%20of%20Cryopreservation%20in%20Fungi.pdf
  • https://europepmc.org/article/med/2797113
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