Fungal Culture Preservation: Introduction, Techniques and Detals About Sterile Water Preservation Technique

Fungal Culture Preservation- The main objectives of fungal culture are to grow the organisms, isolate the organisms, and if necessary preserve the organisms. A fungal culture is a genetic resource and thus fungal culture preservation is applicable for the following purposes; Germplasm conservation Developmental studies Industrial Application Research programs

Fungal Culture Preservation

The main objectives of fungal culture are to grow the organisms, isolate the organisms, and if necessary preserve the organisms. A fungal culture is a genetic resource and thus fungal culture preservation is applicable for the following purposes;

  • Germplasm conservation
  • Developmental studies
  • Industrial Application
  • Research programs

Fungal Culture Preservation Techniques 

Fungal culture preservation techniques are as follows-

  1. Periodic serial subcultures
  2. Sterile water
  3. Sterile mineral oil overlay
  4.  Sterile soil or sand
  5. Silica gel
  6. Drying on organic substrates
  7. L-drying
  8. Mechanical freezers
  9. Lyophilization

Sterile Water Preservation Technique

Sterile Water Preservation Technique is recommended as easy, cheap and satisfactory for preservation of most species of fungi.

Requirements for Sterile Water Preservation Technique

  • Screw cap tubes
  • Distilled water
  • Fungal isolates
  • Inoculating loop/wire
  • Bunsen burner
  • Biosafety cabinet (BSC)
  • Tube rack

Procedure of Sterile Water Preservation Technique

  1. Take  strile  screw cap tube  with sterile distilled water.
  2. Submerge the fungal isolate into a  screw cap tube.
  3. Store it at room temperature (25-28 ° C).

Sub culturing from the water

  1. Clean of the screw caps with 70% ethanol before unscrewing them.
  2. Transfer a fragment from the tube to culture media.
  3. Incubate the culture media at room temperature (24-28° C) between 5-30 days depending on the nature of organisms.
  4. Perform LPCB preparation from culture and you may also use slide cultures for better expression morphological features of fungi.

Note-Only perform these types of activities in biosafety cabinet.

Keynotes

  1. Preservation of fungal cultures in sterile distilled water is also called Castellani’s method that has been endorsed as a method ensuring high rates of fungal viability, purity and stability, low costs and great simplicity where facing economic difficulties.
  2. Numerous fungi will survive for a decade or more in sterile water.
  3. There is no ideal technique of preservation appropriate for all laboratories and applicable to all fungi.
  4. It is noteworthy that all of these approaches are more expensive as compare to sterile water which can significantly reduce culture storage expense.
  5. Liquid nitrogen (LN2) freezers- It is beneficial for following purposes-√Unexcelled for duration of storage

√Long-term viability is maximized

√All types of culturable fungi can be stored.

Further Reading

  • https://hardydiagnostics.com/wpcontent/uploads/2016/05/Saving-fungal-Cultures-Sterile-Water-By-Harris.pdf
  • https://www.researchgate.net/publication/20359358_Preservation_of_fungi_in_water_Castellani_20_years
  • https://www.medigraphic.com/cgi-bin/new/resumenI.cgi?IDARTICULO=47061
  • https://www.biomed.cas.cz/ccbas/Methods%20of%20Cryopreservation%20in%20Fungi.pdf
  • https://europepmc.org/article/med/2797113

 

[134 visitors]

Comments

© 2021 Universe84a.com | All Rights Reserved

1409517

Visitors