Fungal Culture Preservation: Introduction, Techniues and Detals About Ste

Fungal Culture Preservation: Introduction, Techniques and Detals About Sterile Water Preservation Technique

Fungal Culture Preservation

The main objectives of fungal culture are to grow the organisms, isolate the organisms, and if necessary preserve the organisms. A fungal culture is a genetic resource and thus fungal culture preservation is applicable for the following purposes;

Germplasm conservation
Developmental studies
Industrial Application
Research programs

Fungal Culture Preservation Techniques

Fungal culture preservation techniques are as follows-

Periodic serial subcultures
Sterile water
Sterile mineral oil overlay
Sterile soil or sand
Silica gel
Drying on organic substrates
L-drying
Mechanical freezers
Lyophilization

Sterile Water Preservation Technique

Sterile Water Preservation Technique is recommended as easy, cheap and satisfactory for preservation of most species of fungi.

Requirements for Sterile Water Preservation Technique

Screw cap tubes
Distilled water
Fungal isolates
Inoculating loop/wire
Bunsen burner
Biosafety cabinet (BSC)
Tube rack

Procedure of Sterile Water Preservation Technique

Take strile screw cap tube with sterile distilled water.
Submerge the fungal isolate into a screw cap tube.
Store it at room temperature (25-28 ° C).

Sub culturing from the water

Clean of the screw caps with 70% ethanol before unscrewing them.
Transfer a fragment from the tube to culture media.
Incubate the culture media at room temperature (24-28° C) between 5-30 days depending on the nature of organisms.
Perform LPCB preparation from culture and you may also use slide cultures for better expression morphological features of fungi.

Note-Only perform these types of activities in biosafety cabinet.

Keynotes

Preservation of fungal cultures in sterile distilled water is also called Castellani’s method that has been endorsed as a method ensuring high rates of fungal viability, purity and stability, low costs and great simplicity where facing economic difficulties.
Numerous fungi will survive for a decade or more in sterile water.
There is no ideal technique of preservation appropriate for all laboratories and applicable to all fungi.
It is noteworthy that all of these approaches are more expensive as compare to sterile water which can significantly reduce culture storage expense.
Liquid nitrogen (LN2) freezers- It is beneficial for following purposes-√Unexcelled for duration of storage

√Long-term viability is maximized

√All types of culturable fungi can be stored.

universe84a

Fungal Culture Preservation: Introduction, Techniques and Detals About Sterile Water Preservation Technique