Listeria on blood agar: Introduction, Morphology, Culture characteristics, Pathogenesis, Lab Diagnosis and Treatment
Introduction of Listeria
Listeria monocytogenes on blood agar and its colony morphology as shown above image. It is a Gram positive bacterium causes a serious infection called Listeriosis and it is caused by eating food contaminated with the bacterium Listeria Monocytogenes. It is a rod-shaped, intracellular aerobic pathogenic bacterium that invades the cytoplasm of living cells. It develops a distinctive rocket tail structure to help push through the cytoplasm. Eventually, these “rockets” push bacteria into neighboring cells, propagating the infection and it has recently been recognized as an important public health problem in the United States produces but also infections world wide. Important cause of infections in animals and man. The disease affects primarily persons of advanced age, pregnant women, new-born, and adults with weakened immune systems. However, persons without these risk factors can also rarely be affected.
Classification of organism
Species: L. monocytogenes L. dentrificans L. grayi L. innocua L. ivanovii L. seeligeri L. murayi L. welshimeri
Morphology of Listeria
The morphology of genus Listeria are small, Gram positive coccobacilli or rod shaped having size of 1-3µmX 0.5 µm. They are motile at 25°C showing tumbling motility but are non-motile at37°C. They produce peritrichous flagella optimally at 20-30°C, but they can not produce flagella at all. They are non-sporing, non capsulated and acid-fast. They may resemble the morphology of diphtheroids that makes the Microbiologists to identify from Diptheriods, which are mistaken and specimens are discarded.
Culture characteristics of Listeria
L. monocytogenes is an aerobe and facultative anaerobe. It has an unusual feature having capability to grown at refrigeration temperature. The temperature range for growth is 3-45°C. But optimal temperature for growth is 30°C. In blood agar it produces small, grey, translucent drop-like colonies surrounded by a small zone of indistinct beta hemolysis like streptococci but it is catalse test positive. It may require incubation up to 48 hours to produce visible growth. Gram stain is very helpful for checking morphology of colonies.
In Muller-Hinton agar (MHA), colonies appear pale blue-green when viewed from the side ( 45° angle) with a beam of white light.
Biochemical tests of Listeria
Catalase test: Positive
Indole, oxidase and urea hydrolization test: Negative
It ferments glucose and maltose with acid production.
Listeria monocytogenes inoculated at right angles to b-hemolytic Staphylococcus aureus. Note the arrow-shaped zone of weak enhanced hemolysis indicating a positive CAMP test.
Pathogenesis of Listeria
Listeria Monocytogenes enters through the Gastro – intestinal tract after infections of contaminated foods such as cheese or vegetables. The cell wall surface protein called Interanalin interacts with E –cadherin and enters into epithelial cells. Bacteria produce listeriolysin. It can move from cell to with out being exposed to antibodies, complement, polymorphonuclear cells.
Source of Infection
Listeria monocytogenes can be found in a variety of dairy products, vegetables, fish and meat products. Listeria monocytogenes, unlike most other harmful bacteria, will grow slowly on foods stored in a refrigerator. This organism can also be spread by contact with an infected product or surface, such as hands or counter tops, during food preparation.
Prone to Listeriosis are-
People with weakened immune systems
People who are taking immuno-suppressing medication.
Following are the clinical features of Listeriosis-
New- born rather than the pregnant women themselves suffer the serious effects of infection in pregnancy.
Adults may present with bacteremia. Meingoencephalitis occurs most commonly in Immuno-supressed patients in whom Listeria is one of the more common cause of Meningitis. Disease can be insidious to fulminant.
Listeriosis Presenting with Meningitis
Immunocompromised adults are at risk for a serious infection of the blood stream and central nervous system (brain and spinal cord). Meningitis occurs in about half of the cases of adult Listeriosis. Symptoms of listerial meningitis occur about four days after the flu-like symptoms and include fever, personality change, uncoordinated muscle movement, tremors, muscle contractions, seizures, and slipping in and out of consciousness.
Late onset manifestations
The new born child may present with late onset syndrome causes the development of Meningitis between birth and third week of life. It is often caused by serotype IV b and has a significant mortality rate.
Listeriosis and Pregnancy
Pregnant women – They are about 20 times more likely than other healthy adults to get Listeriosis. About one-third of listeriosis cases happen during pregnancy.
Specimens- Blood, CSF, amniotic fluid, pus, swabs from cervical and vaginal secretions, meconium, chord blood, etc.
Direct Microscopy-In Gram stain smears of CSF sediments, the bacteria may be seen as Gram positive coccobacilli.
Culture- Blood agar shows small colonies surrounded by a narrow zone of β-hemolysis as shown above picture.
Biochemical tests-Catalase test: Positive, Indole, oxidase and urea hydrolization test: Negative. It ferments glucose and maltose with acid production and CAMP test: Positive.
Motility test of L. monocytogenes shows tumbling motility.
Treatment of Listeriosis
Listeriosis is a serious disease requiring hospitalization. A combination of antibiotics is given intravenously through a small straw-like catheter. When infection occurs during pregnancy, antibiotics must be given promptly to the mother to prevent infection of the fetus or newborn. The duration of antibiotic treatment is at least two weeks. Even with prompt treatment, some infections result in death. antibacterial drugs in use are-ampicillin, erythromycin, intravenous trimethoprim – sulphmethoxazole (Co-trimoxazole), cephalosporins and fluroquinoles are not active against l.monocytogens. A combination of gentamycin and ampicillin on clinical basis.
Prophylaxis for Listeriosis
Completely cook all meats and eggs. Carefully wash raw vegetables before eating. Keep raw meat away from raw vegetables and prepared foods. After cutting raw meat, wash the cutting board with detergent before using it for vegetables. Avoid drinking unpasteurized milk or foods made from such milk. Wash hands thoroughly after handling raw meat. Follow the instructions on food labels. Observe food expiration dates and storage conditions.
Experimental inoculation of this L. monocytogenes in rabbbits causes a marked monocytosis ( high number of monocytes than normal range in blood) and thus the name monocytogenes.
All the above eight species may cause disease in animals. Only L. monocytogenes and very rarely L. ivanovii to be associated with human disease.
The characteristics motility and cultural characteristics of L. monocytogenes are usually sufficient to identify it without the need to use many biochemical tests.
Bacteria Erysipelothrix rhusiopathiae, L. monocytogenes and Corynebacterium species can be differentiated on the basis of motility test, catalase test and production of hydrogen sulphide. Among them, Erysipelothrix rhusiopathiae only produces hydrogen sulphide (H2S) while L. monocytogenes is only motile. Corynebacterium species is negative for those tests.
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