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Introduction of Trichomonas vaginails saline smear Trichomonas vaginails saline smear is useful to identify the causative agent of venereal disease, trichomoniasis. Trichomoniasis is a sexually transmitted infection. A protozoan parasite, Trichomonas vaginalis is a causative agent of this infection that is very common. and easily treatable. T.  vaginalis travels from person to person through genital contact during sex. In women, the organism causes an infection in the vagina, urethra, or both. In men, the infection only happens in the urethra. Once...

Introduction of K pneumoniae Klebsiella is from the surname of  German-Swiss microbiologist Edwin Klebs (1834–1913). Klebsiella is a genus of Gram-negative, oxidase-negative, fimbriated, non -motile, non-sporing rod-shaped bacteria with a prominent polysaccharide-based capsule and having size o,f   0.5 -0.8 µm wide to 1 -2  µm. They are found everywhere in nature and also in bacterial flora in our intestines. K pneumoniae scientific classification is as follows Domain: Bacteria Phylum: Proteobacteria Class: Gammaproteobacteria Order: Enterobacterales Family: Enterobacteriaceae Genus: Klebsiella Species:  pneumoniae Subspecies...

Introduction of Oxidase Test  (Kovac’s Method)  Oxidase test negative and positive as shown above picture. Gordon and McLeod introduced the use of a dimethyl-phenylenediamine dihydrochloride solution to test for the presence of oxidase systems in 1928. In particular, they used the test to distinguish N. gonorrhoeae (oxidase test positive) from Staphylococcus species and Streptococcus species (oxidase negative). The sensitivity of the oxidase test was increased when Kovács found that a tetramethyl-p-phenylenediamine dihydrochloride solution gave a quicker reaction. Gaby and Hadley developed a...